01-02-2022, 02:29 PM
A bunch of nothingness with no evidence.
“If you want to know who rules over you, just look for who you are not allowed to criticize.”
― Voltaire
― Voltaire
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Rogan & Malone
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01-03-2022, 09:29 AM
Speaking of nothingness. Still haven't seen anything about Malone inventing anything
01-03-2022, 11:02 AM
You must be fun at parties when you make a total ass of yourself.
Compounds and methods for enhancing delivery of free polynucleotide Publication number: 20040009947 Abstract: The discovery of simple, nontoxic, and pharmaceutically defined methods for genetic modification of cells and tissues would enable development of a variety of molecular medicines. “Free”, ‘direct’, or ‘naked’ polynucleotide administration is a simple, apparently safe, and pharmaceutically defined polynucleotide delivery method. Murine, macaque, and clinical human experiments have demonstrated transfection of various tissues, such as respiratory tissues, after direct application of ‘free’ polynucleotide. However, direct DNA transfection is relatively inefficient in comparison to many transduction systems. The invention herein is directed to transfection enhancing agents which augment the transfection activity of ‘free’ polynucleotide, thereby facilitating the development of simple and safe alternatives to tissue transfection, more particularly respiratory tissue transfection. Type: Application Filed: May 27, 2003 Publication date: January 15, 2004 Inventors: Robert W. Malone, Jill G. Malone DNA vaccines for eliciting a mucosal immune response Patent number: 6110898 Abstract: The invention consists of a method for inducing production of a mucosal immune response in a host by administration of an antigen-encoding polynucleotide preparation, comprising DNA or RNA encoding an antigenic epitope to a mucosal inductor site in the mucosal tissue of the host. Naked DNA may be administered directly to mucosa, for instance in saline drops, or in a recombinant gene expression vector. Preferably, the recombinant gene expression vectors are not capable of replication or dessimination. The invention also includes the use of live viral vaccines wherein the viruses include immunostimulatory polynucleotides of the invention. According to a preferred method of the invention, a target protein antigen is administered through its expression by a recombinant gene expression vector. Type: Grant Filed: May 23, 1997 Date of Patent: August 29, 2000 Assignee: University of Maryland, Baltimore Inventors: Robert W. Malone, Jill G. Malone Formulations and methods for generating active cytofectin: polynucleotide transfection complexes Patent number: 5925623 Abstract: In the generation of cytofectin:polynucleotide complexes for transfection of cells, formulations, counterions, and reaction conditions for maximizing the transfection include using a cationic amine compound that has the general structure: ##STR1## wherein R.sub.4 and R.sub.5 are a pair of same or different lipoyl moieties selected from a group consisting of an alkyl, alkenyl, alkynyl, alkanoyl, alkenoyl, or alkynoyl groups and for R.sub.1, R.sub.2, and R.sub.3 at least two are hydroxylated, ether containing, or acyloxy containing alkyl, alkenyl, or alkynyl groups or at least one amine bonded halogen containing moiety selected from a group consisting of a halogenated alkyl, alkenyl, or alkynyl group or a mixture of at least one halogen containing moiety selected from a group consisting of a halogenated alkyl, alkenyl, or alkynyl group and at least one hydroxylated, ether containing, or acyloxy containing alkyl, alkenyl, or alkynyl group, and X.sup.- is an oxyanion or halide counterion. Type: Grant Filed: July 15, 1996 Date of Patent: July 20, 1999 Assignee: The Regents of the University of California Inventors: Michael H. Nantz, Michael J. Bennett, Rajiv P. Balasubramaniam, Alfred M. Aberle, Robert W. Malone Cationic transport reagents Patent number: 5892071 Abstract: For use in transporting biologically active species into and through membrane barriers, a symmetrical cationic diamine compound having the general structure ##STR1## wherein m=1-10; R.sub.1 is a hydrogen, an alkyl group, an alkenyl group, a hydroxylated alkyl or alkenyl group, or an ether containing alkyl or alkenyl group; R.sub.2 is an alkyl group, an alkenyl group, or an alkyl or alkenyl containing acyl group; R.sub.3 is a hydrogen, an alkyl group, an alkenyl group, a hydroxylated alkyl or alkenyl group, or an ether containing alkyl or alkenyl group; R.sub.4 is a hydrogen, an alkyl group, an alkenyl group, a hydroxylated alkyl or alkenyl group, or an ether containing alkyl or alkenyl group; and X.sup.- is an anion. Type: Grant Filed: September 15, 1995 Date of Patent: April 6, 1999 Assignee: The Reagents of The University of California Inventors: Michael H. Nantz, Michael J. Bennett, Robert W. Malone Polyfunctional cationic cytofectins, formulations and methods for generating active cytofectin: polynucleotide transfection complexes Patent number: 5824812 Abstract: Amine containing compounds and their use in the generation of cytofectin:polynucleotide complexes for transfection of cells, formulations, counterions, and reaction conditions for maximizing the transfection include using cationic amine compounds that have the general structure: ##STR1## wherein R.sub.4 and R.sub.5 are a pair of same or different lipoyl moieties selected from a group consisting of an alkyl, alkenyl, alkynyl, alkanoyl, alkenoyl, or alkynoyl groups and for R.sub.1, R.sub.2, and R.sub.3 at least two are hydroxylated, ether containing, or acyloxy containing alkyl, alkenyl, or alkynyl groups or at least one amine bonded halogen containing moiety selected from a group consisting of a halogenated alkyl, alkenyl, or alkynyl group or a mixture of at least one halogen containing moiety selected from a group consisting of a halogenated alkyl, alkenyl, or alkynyl group and at least one hydroxylated, ether containing, or acyloxy containing alkyl, alkenyl, or alkynyl group, and X.sup. Type: Grant Filed: September 17, 1996 Date of Patent: October 20, 1998 Assignee: The Regents of the University of California Inventors: Michael H. Nantz, Michael J. Bennett, Rajiv P. Balasubramaniam, Alfred M. Aberle, Robert W. Malone Cationic transport reagents Patent number: 5744625 Abstract: For use transporting biologically active species into and through membrane barriers, a symmetrical cationic diamine compound having the general structure ##STR1## Type: Grant Filed: May 16, 1996 Date of Patent: April 28, 1998 Assignee: The Reagents of the University of California Inventors: Michael H. Nantz, Michael J. Bennett, Robert W. Malone Induction of a protective immune response in a mammal by injecting a DNA sequence Patent number: 5589466 Abstract: A method for delivering an isolated polynucleotide such as DNA or RNA, to the interior of a cell in a mammal comprising the injection of an isolated polynucleotide into a muscle of the mammal where the polynucleotide is taken up by the cells of the muscle and exerts a therapeutic effect on the mammal. The method can be used to deliver a therapeutic polypeptide to the cells of the mammal, to provide an immune response upon in vivo translation of the polynucleotide, to deliver antisense polynucleotides, to deliver receptors to the cells of the mammal or to provide transitory gene therapy. Type: Grant Filed: January 26, 1995 Date of Patent: December 31, 1996 Assignees: Vical Incorporated, Wisconsin Alumni Research Foundation Inventors: Philip L. Felgner, Jon A. Wolff, Gary H. Rhodes, Robert W. Malone, Dennis A. Carson Delivery of exogenous DNA sequences in a mammal Patent number: 5580859 Abstract: Polynucleotide sequences, comprising DNA and RNA molecules can be directly administered, for example by injection, to tissues, such as muscle, and expressed as a protein, polypeptide or polypeptide. The polynucleotides can be contained within liposomes or the polynucleotides can free from association with transfection-facilitating proteins, viral particles, liposomal formulations, charged lipids and calcium phosphate precipitating agents. Type: Grant Filed: March 18, 1994 Date of Patent: December 3, 1996 Assignees: VICAL Incorporated, Wisconsin Alumni Research Foundation Inventors: Philip L. Felgner, Jon A. Wolff, Gary H. Rhodes, Robert W. Malone, Dennis A. Carson Cationic transport reagents Patent number: 5527928 Abstract: For use in transporting biologically active species into and through membrane barriers, a symmetrical cationic diamine compound having the general structure ##STR1## Type: Grant Filed: September 30, 1994 Date of Patent: June 18, 1996 Inventors: Michael H. Nantz, Michael J. Bennett, Robert W. Malone How ridiculous that you didn't read this when it was posted @ #7
“If you want to know who rules over you, just look for who you are not allowed to criticize.”
― Voltaire
01-03-2022, 11:15 AM
Yes. I'm a barrel of monkeys at a party.
But still waiting for anybody, aside from Malone himself, to claim thqt he actually invented mrna vaccines
01-03-2022, 11:30 AM
Quote:But still waiting for anybody, aside from Malone himself, to claim thqt he actually invented mrna vaccinesBecause you aren't the sharpest knife in the drawer, I'll help you out" 1. Find a search engine 2. Type "Robert Malone + Mrna vaccine" into the search box 3. Find the button that says 'Search" 4. Click "Search" 5. Wait for the "Results" 6. Count the results 7. After counting the results, Get Back To Me with that number. 8. You now have your answer.
“If you want to know who rules over you, just look for who you are not allowed to criticize.”
― Voltaire
01-03-2022, 11:46 AM
Let's have the link to that page
“If you want to know who rules over you, just look for who you are not allowed to criticize.”
― Voltaire
01-03-2022, 12:16 PM
You don't even follow instructions worth a shit.
Regardless, do you think you could find a magazine where Pfizer is not one of their advertisers.
“If you want to know who rules over you, just look for who you are not allowed to criticize.”
― Voltaire |
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